Video: Restriction Enzyme Digests: Principle, Procedure, and Applications
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Video: Restriction Enzyme Digests: Principle, Procedure, and Applications
305.6K Views. Restriction enzymes or endonucleases recognize and cut DNA at a specific sequence. These enzymes occur naturally in bacteria as a defense against bacteriophages - viruses that infect bacteria. Bacterial restriction enzymes cut the invading bacteriophage DNA while leaving the bacterial genomic DNA unharmed due to addition of methyl ...
302.4K viewsFeb 1, 2013
Restriction enzyme DNA Cutting
'Restriction' in Restriction enzyme refers to(1) cleaving of ph... | Filo
'Restriction' in Restriction enzyme refers to(1) cleaving of ph... | Filo
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6 months ago
EcoRI cutting a strand of DNA. EcoRI is a restriction endonuclease that recognizes and cuts DNA at a specific motif (GAATTC). After the DNA is cut, so-called sticky ends are left behind. Sticky ends are short non-paired DNA bases. This property makes this enzyme a powerful tool in molecular biology. The enzyme naturally occurs in E. coli, where it serves as a defense mechanism against viral DNA. | Ribosome Studio
0:20
EcoRI cutting a strand of DNA. EcoRI is a restriction endonuclease that recognizes and cuts DNA at a specific motif (GAATTC). After the DNA is cut, so-called sticky ends are left behind. Sticky ends are short non-paired DNA bases. This property makes this enzyme a powerful tool in molecular biology. The enzyme naturally occurs in E. coli, where it serves as a defense mechanism against viral DNA. | Ribosome Studio
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